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jackson immunoresearch cat  (Jackson Immuno)


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    Jackson Immuno jackson immunoresearch cat
    Jackson Immunoresearch Cat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 190 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jackson+immunoresearch+cat/Cy+3+AffiniPure+F(ab')%E2%82%82+Fragment+Donkey+Anti-Mouse+IgG/pm41932651-118-25-25
    Average 95 stars, based on 190 article reviews
    jackson immunoresearch cat - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: Targeting AT 1 receptors with telmisartan protects SH-SY5Y cells from 6-OHDA induced Parkinsonian neurotoxicity.
    Article Snippet: After fixation, the cells were blocked in 5% BSA for 60 minutes after permeabilized with PBS containing 0.1% Triton-X-100 for 10 minutes. . Then, the samples were incubated with primary antibodies against α-synuclein (Santa Cruz, Cat. no. sc-69977) and IL-1β (Santa Cruz, Cat. no. sc-52012) (1:200) overnight at 4 °C. .. The cells were then washed three times with PBS and incubated at room temperature for 1 h with a goat anti-mouse FITC-conjugated secondary antibody (Jackson ImmunoResearch, Cat. No. 115–095–003) at dilution ratios of 1:100, 1:200, ..

    Article Title: FCRN/antigen-binding molecules and methods of use
    Article Snippet: .. Samples were incubated for 1 h and detected by an HRP-conjugated goat anti-mouse Fab2 (HRP Peroxidase AffiniPure F(ab′)2 Fragment Goat Anti-Mouse IgG, F(ab′)2 Fragment Specific, Jackson ImmunoResearch, Cat. 115-036-072) for 1 h on a shaking incubator. ..

    Article Title: Molecular tension indicators reveal unexpectedly complex regulation of tension in live mouse organs
    Article Snippet: .. After permeabilization with 0.1% Triton (Nacalai, cat. 35501-15) in PBS (“PBSTr”, hereafter) at room temperature (RT) for 10 min, sections were subjected to blocking with 10% donkey serum, 3% skim milk, AffiniPure Fab Fragment Goat Anti-Mouse IgG (H + L) (1:100, Jackson ImmunoResearch, cat. 115-007-003) in PBSTr at RT for 1 h. After rinse with PBSTr twice, sections were incubated with primary antibodies at 4 °C overnight. .. After washing in PBSTr at RT for 10 min for three times, sections were incubated with secondary antibodies, Hoechst 33342 (1 mg/mL, 1:100), with or without phalloidin, at RT for 90 min. After washing in PBSTr at RT for 10 min three times, sections were mounted with fluorescence mounting medium (Agilent Dako, cat S3023).

    Article Title: Hypoxia-induced gene expression changes in N. vectensis embryos.
    Article Snippet: Independently fixed N. vectensis258 embryos were blocked in PBST + 1% BSA for 1hr at RT, and then incubated with 1:200 Phospho-Histone H3 (Ser10) Antibody259 (Cell Signalling, cat. 9701) in PBST + 1% BSA overnight at 4oC. .. A solution of Goat anti-rabbit IgG secondary antibody260 (1:1000) (Jackson ImmunoResearch, cat. 111-545-006) and DAPI (1:2000) (Sigma-Aldrich, cat. MBD0015-1ML) in PBST +261 1% BSA was added to the embryos, which were then incubated for 1hr at RT in the dark. ..

    other:

    Article Title: The hypothalamus is an early site of mitochondrial failure and neuro-immune circuit disruption in amyotrophic lateral sclerosis.
    Article Snippet: Secondary antibodies included Donkey anti-Rat IgG (H+L) Alexa Fluor 488 (Thermo Fisher Scientific, Cat. #A21208, 1:200) and Cy3 AffiniPure F(ab′)2 Fragment Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, Cat. #715-166- 150, 1:100).

    Article Title: Structure of SHOC2-KRAS-PP1C complex reveals RAS isoform-specific determinants and insights into targeting complex assembly by RAS inhibitors
    Article Snippet: Anti-Mouse IgG (light chain) antibody Alexa Fluor 680 , 1:15,000 , Jackson ImmunoResearch Cat.# 115-625-174.

    Article Title: Structure of SHOC2-KRAS-PP1C complex reveals RAS isoform-specific determinants and insights into targeting complex assembly by RAS inhibitors.
    Article Snippet: Table 2: Antibodies used in this study Antibodies Dilution Source Myc-tag Rabbit polyclonal Ab 1:2000 Cell Signaling Technology Cat.# 2272 PP1 alpha Rabbit polyclonal Ab 1:2000 Upstate Cat.# 06-221 FLAG Rabbit polyclonal Ab 1:4000 Sigma-Aldrich Cat.# F7425 CRAF Mouse mAb 1:2000 BD Biosciences Cat.# 610152 P-S259 RAF1 Rabbit polyclonal Ab 1:2000 Cell Signaling Technology Cat.# 9421 Anti-Rabbit IgG (H&L) Antibody DyLight 800 1:15,000 Invitrogen Cat.# SA5-35571 Anti-Mouse IgG (light chain) antibody Alexa Fluor 680 1:15,000 Jackson ImmunoResearch Cat.# 115-625174 Data availability The atomic coordinates and structure factors have been deposited in the Protein Data Bank and can be accessed using accession numbers 9O65 [http://doi.org/10.2210/pdb9O65/pdb], EMD70159 [https://www.ebi.ac.uk/emdb/EMD-70159] (stabilized SKP complex), 9O0N [http://doi.org/10.2210/pdb9O0N/pdb] (KRAS(1-169)GDP with MRTX1133), 9O0O [http://doi.org/10.2210/pdb9O0O/pdb] (KRAS (1-169)GMPPNP with MRTX1133), 9O0P [http://doi.org/10.2210/pdb9O0P/pdb] (MRASmut(1-178)GDP with MRTX1133) and 9O0Q [http://doi.org/10.2210/pdb9O0Q/pdb] (MRASmut(1-178)GMPPNP with MRTX1133).

    Blocking Assay:

    Article Title: Molecular tension indicators reveal unexpectedly complex regulation of tension in live mouse organs
    Article Snippet: .. After permeabilization with 0.1% Triton (Nacalai, cat. 35501-15) in PBS (“PBSTr”, hereafter) at room temperature (RT) for 10 min, sections were subjected to blocking with 10% donkey serum, 3% skim milk, AffiniPure Fab Fragment Goat Anti-Mouse IgG (H + L) (1:100, Jackson ImmunoResearch, cat. 115-007-003) in PBSTr at RT for 1 h. After rinse with PBSTr twice, sections were incubated with primary antibodies at 4 °C overnight. .. After washing in PBSTr at RT for 10 min for three times, sections were incubated with secondary antibodies, Hoechst 33342 (1 mg/mL, 1:100), with or without phalloidin, at RT for 90 min. After washing in PBSTr at RT for 10 min three times, sections were mounted with fluorescence mounting medium (Agilent Dako, cat S3023).

    Article Title: Molecular tension indicators reveal unexpectedly complex regulation of tension in live mouse organs.
    Article Snippet: .. After permeabilization with 0.1% Triton (Nacalai, cat. 35501-15) in PBS (“PBSTr”, hereafter) at room temperature (RT) for 10min, sections were subjected to blocking with 10% donkey serum, 3% skim milk, AffiniPure Fab Fragment Goat Anti-Mouse IgG (H+L) (1:100, Jackson ImmunoResearch, cat. 115-007-003) in PBSTr at RT for 1hr. ..



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